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KMID : 0545120030130020309
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Journal of Microbiology and Biotechnology
2003 Volume.13 No. 2 p.309 ~ p.312
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Characteristics of Nitrobenzene Degradation by Mycobacterium chelonae Strain NB01
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OH, YOUNG-SOOK
LEE, YOUN-HEE/LEE, JUNG-HYUN/CHOI, SUNG-CHAN
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Abstract
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A bacterial strain NB01, isolated from wastewater, was found to utilize nitrobenzene (NB) as the sole source of nitrogen, carbon, and energy. The strain was classified as a member of a high G+C Gram-positive group and identified as Mycobacterium chelonae based on an analysis of its 16S rDNA gene sequence. The strain grew on NB with a concomitant release of about 63% of the total available nitrogen as ammonia, suggesting a reductive degradation mechanism. The optimal pH and temperature for degradation were pH 7.0-8.0 and 30℃, respectively. The cell growth was retarded at NB concentrations above 1.8 mM. The degradation of NB followed Michaelis-Manten kinetics within the tolerance range, and the K_m and maximum specific removal rate for NB were 0.33 mM and 11.04 h^-1, respectively.
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KEYWORD
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